Coding
Part:BBa_K3972000:Design
Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen Group: iGEM21_TU-Eindhoven (2021-09-20)
TtrS
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1561
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1561
Illegal NheI site found at 1309
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1561
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1561
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1561
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408
Illegal AgeI site found at 783 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Only Type IIS BioBrick compatible.
Source
From plasmid pKD227 ordered from Addgene.