Coding

Part:BBa_K3972000:Design

Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen   Group: iGEM21_TU-Eindhoven   (2021-09-20)


TtrS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1561
    Illegal PstI site found at 1013
    Illegal PstI site found at 1028
    Illegal PstI site found at 1084
    Illegal PstI site found at 1381
    Illegal PstI site found at 1408
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1561
    Illegal NheI site found at 1309
    Illegal PstI site found at 1013
    Illegal PstI site found at 1028
    Illegal PstI site found at 1084
    Illegal PstI site found at 1381
    Illegal PstI site found at 1408
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1561
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1561
    Illegal PstI site found at 1013
    Illegal PstI site found at 1028
    Illegal PstI site found at 1084
    Illegal PstI site found at 1381
    Illegal PstI site found at 1408
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1561
    Illegal PstI site found at 1013
    Illegal PstI site found at 1028
    Illegal PstI site found at 1084
    Illegal PstI site found at 1381
    Illegal PstI site found at 1408
    Illegal AgeI site found at 783
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Only Type IIS BioBrick compatible.


Source

From plasmid pKD227 ordered from Addgene.

References